clinical reagent Tuberculosis TB STD rapid test Strip

Tuberculosis(TB)-IgG antibody

Rapid Gold Immuno-assay Kit for Antibody IgG to Tuberculosis (TB) is an in vitro immunoassay for the qualitative determination of Anti-TB antibody IgG in human serum or plasma in one step. It is for diagnosis of early infection and epidemic survey.

The kit uses one step method to detect anti-TB antibodies IgG in serum or plasma. The purified specific recombinant TB antigen is immobilized on the nitrocellulose membrane at the test line region and another specific TB Ag conjugated to the colloidal gold is coupled on the label pad. To perform the test, a sample is added to the sample well of the test card. The sample flows through to the label pad. If the sample contains Antibody IgG to Tuberculosis, the antibody will bind to the antigen coated on the colloidal gold particles to form antibody-antigen-gold complexes. These complexes move on the nitrocellulose membrane by capillary action toward the test line region, and react with specific recombinant TB antigen to produce a pink color on the membrane. A second red control line will always appear in the result window to indicate that the test has been correctly performed and the test device functions properly. If Antibody IgG to Tuberculosis (TB) is not present or lower than the detection limit of the test, only the control line will be visible. If the control line dose not developed, the test is invalid. The pink color intensity will reflect the antibody intensity in the sample. The test is fast and easy to operate.

Antibody IgG to Tuberculosis test card/strip

Each cassette/strip contains a test strip with TB antigen on the test region of the membrane and colored Tuberculosis antigen-gold conjugate pad.

Blood serum samples are routinely prepared form vein. Blood plasma sample are routinely prepared with routine amount of anticoagulant such as heparin or sodium citrate. Samples may be stored at 2-8°C within five days without interfering with the assay performance. For long-term storage of specimens, -20°C or colder is recommended. Repeated freezing and thawing of specimens is not recommended and may cause erroneous results. Samples with cloud or precipitation should be centrifugated or filtered before test. Prevent serum from bacteria contamination during collection and storage.

The testing is for qualitative and assistant diagnosis. Confirmation of infection should refer to the clinical and other diagnosis.

1. 1. Both plate and sample should be with room temperature 20-25°C
2. 2. Test sample must be fresh.
3. 3. The result after 20 minutes is invalid.
4. 4. Diluted sample may generate false positive result
5. 5. Bacteria contamination, serious hemolysis, jaundice and high lipid blood will cause wrong result. The sample should be re-collected.
6. 6. Wrong result might be caused by expired kit or problematic samples.

40 tests/Kit